Data/hora: 16/05/2024 08:28:59

Provedor de dados: 	45	
País: 	Brazil	
Título: 	Validation of an HPLC-UV method for the identification and quantification of bioactive amines in chicken meat	
Autores: 	Assis,D.C.S.; Menezes,L.D.M.; Lima,A.L.; Klein,R.W.T.; Heneine,L.G.D.; Lara,L.J.C.; Teixeira,L.V.; Ornellas,C.B.D.; Cançado,S.V.; Figueiredo,T.C.	
Data: 	2016-06-01	
Ano: 	2016	
Palavras-chave: 	Validation; Chicken meat; Bioactive amines; HPLC-UV.	
Resumo: 	ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1, with a limit of detection at 0.3mg.kg-1 and a limit of quantification at 0.9mg.kg-1 for all amines, with the exception of histamine, which exhibited the limit of quantification, of 1mg.kg-1. In conclusion, the performance parameters demonstrated adequacy of the method for the detection and quantification of bioactive amines in chicken meat.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300805	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/1678-4162-8668	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.68 n.3 2016	
Fonte: 	info:eu-repo/semantics/openAccess